Generation of a <i>Pdss2</i> Conditional Null Allele.

<p>Generation of a <i>Pdss2</i> conditional null allele, showing a map of the <i>Pdss2</i> genomic locus and the targeting vector with exons represented by open boxes. The relative position of PCR primers (small arrowheads), loxP (large arrowheads), as well as cassettes encoding neomycin phosphotransferase (neo) are shown. Primers koF, koF2 and koR were used in PCR genotype analysis. Cre-mediated deletion results in either the <i>Pdss2</i> null allele (deletion of exon 3 ) or the <i>Pdss2 ^loxP/loxP</i> allele (exon 2 flanked by loxP sites). Abbreviations: Ba, BamH I; Aa. Aat II; Xh, Xho I; Dr, Dra III.