Suppression of HIV-1 replication in infected MDDC by the CD85j⁺ NK cell subset.

<p>(a) NKR expression on purified CD85j⁺ and CD85j⁻ NN cell subsets. Results are expressed as percentage of receptor expression in the given subset+SE. (b) MDDC were infected with HIV-1_Bal and then cultured alone (DC) or incubated with total NK cells (NK) or with the CD85j⁺ or CD85j⁻ NK cell subsets. HIV-1 replication was measured by p24 production at the indicated time points. Mean+SE of p24 levels in 8 independent experiments using cells from different donors. p24 levels in NK/MDDC cocultures were compared to those in MDDC cultures * p<0.02, **p<0.001. (b) MDDC were one-round infected with HIV-1/BaL pseudotypes and then cultured alone (DC) or incubated with total NK cells (NK) or with the CD85j⁺ or CD85j⁻ NK cell subsets. Ninety-six hours later, cells were lysed and luciferase activities were measured. Results are expressed as means±SE of luciferase activity of 4 independent experiments. CD85j⁺ NK cells induced higher levels of inhibition than CD85j⁻ NK cells. * p<0.05