Supercomplex organisation of OXPHOS complexes in PBMC and neutrophils.

<p>A–B) Mitochondria isolated from PBMC (A) and neutrophils (B) were solubilized in digitonin, and 200 µg of mitochondrial protein was separated by BN-PAGE. Strips from the first dimension were excised and used for second dimension SDS-(12%)PAGE, transferred to PVDF membrane and immuno-detected with anti-complex I (20 kDa), complex II (30 kDa), complex III (Core 2, 47 kDa), complex IV (COX II, 26 kDa) and complex V (α, 55 kDa) subunit antibodies. Blots are representative of three independent experiments. High molecular weight purified proteins were used as molecular mass markers in the first dimension BN-PAGE: thyroglobulin, 669 kDa; ferritin monomer, 440 kDa; catalase 232 kDa; lactate dehydrogenase, 140 kDa.