Mouse infections with RNA viruses.

a<p>1 day post-infection was reported to correspond to the peak of LDV replication and of IFN expression in vivo.</p>b<p>Mice infected with highly neurovirulent viruses were sampled when signs of encephalitis were prominent (generally less than 24 hours before death).</p>c<p>The DA1 strain of TMEV produces a transient encephalitis lasting about 1 or 2 weeks. In mice with the H-2 b haplotype, the virus is then rapidly cleared by the cytolytic T lymphocyte response <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1000017#ppat.1000017-Brahic1" target="_blank">[40]</a>,<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1000017#ppat.1000017-Roos1" target="_blank">[41]</a>. Mice were sampled at 5 days post-infection, a time-point representative of the acute phase of infection.</p>d<p>Preliminary RT-PCR experiments failed to reveal a clear difference in IFN expression and viral load between 129/Sv mice infected i.p. for 2 days and for 7 days. Only mice with amounts of MHV-A59 detectable by conventional RT-PCR were taken into account.</p>e<p>These samples from TMEV (GDVII strain) and LACVdelNSs infected brains were from a previous work <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1000017#ppat.1000017-Delhaye1" target="_blank">[33]</a>.