Mated and Naive Females Prefer Unfamiliar Directed Songs to Unfamiliar Undirected Songs

<div><p>(A) Mean percent of time that mated females spent in the unfamiliar undirected and directed song chambers for the four behavior tests on which females displayed a preference. Females significantly preferred unfamiliar directed to undirected songs (*<i>p</i> < 0.05).</p> <p>(B) Mean percent of time that naive females spent in the unfamiliar undirected and directed song chambers. Seven females showed significant preferences for at least one of the stimuli. Because some females responded to stimuli from more than one male, diamonds represent the average response for each female. Naive females significantly preferred directed song to undirected song (*<i>p</i> < 0.05).</p> <p>(C) Mated females tested on their mate's songs spent significantly more time in the directed song chamber (*<i>p</i> < 0.05) than did naive or mated females tested on unfamiliar songs.</p> <p>(D) Number of undirected and directed song bouts produced over a 6-h period by males housed with their mates. While directed song bouts tended to be longer and accompanied by additional courtship behaviors and copulation attempts, males sang significantly more undirected song bouts (*<i>p</i> < 0.05).</p>