Peptide binding affinity of biotinylated HLA-A*0201 versus non-biotinylated.

<p>The peptide binding affinities of the biotinylated HLA-A*0201-HAT-BSP was compared to the standard truncated HLA-A*0201 molecule. The HLA-A*0201 peptide binding affinities of a panel of pox peptides ranging from god binders (0–10 nM) over intermediary (10–100 nM) to non-binders (>1 µM) were determined in a quantitative ELISA <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0001678#pone.0001678-SylvesterHvid1" target="_blank">[14]</a>. The HC were folded with various concentrations of peptide and excess of hβ₂m. The K_D values for each peptide MHC combination were calculated and the results presented as means of two independent experiments with the corresponding confidence intervals.