Multiple subsets of DC present influenza HA antigen to T cells in BALB/c mice infected with PR8 virus by s.c. injection into the footpad.

<p>The popliteal lymph nodes were isolated and non-DC lineages were removed by antibody depletion. CD11c⁺ DC subsets were purified by flow cytometric sorting into DN DC, CD8α DC and pDC before culturing titrating numbers of each subset with 5×10⁴ CFSE-labelled influenza-specific CD8⁺ T cells (<i>A,C</i>) or CD4⁺ T cells (<i>B,D</i>) 48 (<i>A,B</i>) and 72 (<i>C,D</i>) h after infection. The x-axis shows the range of DC used as stimulators over different experiments. Proliferation was analysed at 60 h of culture. Data are pooled from (<i>A–C</i>) three and four (<i>D</i>) independent experiments and show the mean and SEM. Significant differences (<i>p</i>≤0.05) in proliferation induced by different DC populations are indicated by an asterisk.