<p>Levels of proteins in non-transfected, transfected with siRNA control, or with siRNA specific for VRK1 were determined in after 120 hours post transfection. The proteins were determined with the corresponding antibody indicated in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0001642#s4" target="_blank">methods</a> section, and identified in the corresponding immunoblot. These experiments were independently performed five times.</p
<p>An siRNA library targeting 710 human protein kinases was screened in MCF-7 cells, and the normali...
<p>A) Correlations between α-adducin (<i>left panel</i>) and γ-adducin (<i>right panel</i>) and SIK2...
<p>Multiple cancer cell lines were transfected in a high-throughput format with libraries of siRNA c...
<p>(A). Proliferation of WS1 cells that were transfected with siRNA control or siRNA specific for VR...
<p>A. Histograms showing cell cycle distribution after siWee1 transfection for 48 hours, measured by...
<p>(A) The activation levels of PI3K, AKT, and mTOR determined by western blotting. A549 cells were ...
<p>(A). Flow cytometry profile of WS1 cell cycle in non-synchronized (left) and serum starved cells ...
<p>(A) The variation in levels of endogenous VRK1 and p53 proteins in HeLa cells was followed at dif...
<p><b>A)</b> Cells were transfected without siRNA, with scramble siRNA (scramb) or with siRNA agains...
<p>(A). Effect of shRNA on VRK2 endogenous levels in HeLa cells. HeLa cells were transfected as indi...
<p>(A). HeLa cells were transfected with three shRNA constructs specific for human VRK2 or the mixtu...
<p>MDA-MB-435S cells were left untreated or transfected with control or Src siRNA. 24 hours later, s...
BACKGROUND: In mammalian cells regulatory proteins controlling the cell cycle are necessary due to t...
<p>(A) T47D cells were infected with lentivirus particles that expressed Vav1-specific shRNA or shRN...
Cells were transfected with DLL1-siRNA1 or Ctr-siRNA. (A) At 55–70 hours following transfection tota...
<p>An siRNA library targeting 710 human protein kinases was screened in MCF-7 cells, and the normali...
<p>A) Correlations between α-adducin (<i>left panel</i>) and γ-adducin (<i>right panel</i>) and SIK2...
<p>Multiple cancer cell lines were transfected in a high-throughput format with libraries of siRNA c...
<p>(A). Proliferation of WS1 cells that were transfected with siRNA control or siRNA specific for VR...
<p>A. Histograms showing cell cycle distribution after siWee1 transfection for 48 hours, measured by...
<p>(A) The activation levels of PI3K, AKT, and mTOR determined by western blotting. A549 cells were ...
<p>(A). Flow cytometry profile of WS1 cell cycle in non-synchronized (left) and serum starved cells ...
<p>(A) The variation in levels of endogenous VRK1 and p53 proteins in HeLa cells was followed at dif...
<p><b>A)</b> Cells were transfected without siRNA, with scramble siRNA (scramb) or with siRNA agains...
<p>(A). Effect of shRNA on VRK2 endogenous levels in HeLa cells. HeLa cells were transfected as indi...
<p>(A). HeLa cells were transfected with three shRNA constructs specific for human VRK2 or the mixtu...
<p>MDA-MB-435S cells were left untreated or transfected with control or Src siRNA. 24 hours later, s...
BACKGROUND: In mammalian cells regulatory proteins controlling the cell cycle are necessary due to t...
<p>(A) T47D cells were infected with lentivirus particles that expressed Vav1-specific shRNA or shRN...
Cells were transfected with DLL1-siRNA1 or Ctr-siRNA. (A) At 55–70 hours following transfection tota...
<p>An siRNA library targeting 710 human protein kinases was screened in MCF-7 cells, and the normali...
<p>A) Correlations between α-adducin (<i>left panel</i>) and γ-adducin (<i>right panel</i>) and SIK2...
<p>Multiple cancer cell lines were transfected in a high-throughput format with libraries of siRNA c...