The effect of ghrelin and cannabinoids on food intake, hypothalamic AMPK activity and endocannabinoid content.

<p>(A) Cumulative food intake of WT and CB1-KO mice after 2 hours of treatment with icv ghrelin (G) or vehicle (C), n = 5–9 mice/group. (B) Ghrelin and cannabinoid effects on hypothalamic AMPK activity one hour after intraperitoneal administration of THC, ghrelin, rimonabant (R) or a combination of rimonabant and ghrelin (R+G) in WT and CB1-KO animals compared to vehicle-treated animals, n = 6–14 mice/group. (C) Hypothalamic 2-AG content after intraperitoneal treatment with ghrelin in WT and CB1-KO animals, n = 6 mice/group. (D) Hypothalamic 2-AG content in WT animals after ip treatment with ghrelin and rimonabant compared to vehicle treated animals, n = 6–14 mice/group. (E) Hypothalamic AEA content after ip treatment with ghrelin in WT and CB1-KO animals. All data shown as mean±SEM, *P<0.05, **P<0.01, ***P<0.001, ⁺P = 0.052.