X-chromosome Inactivation in E7.5 PGCs Is Random

<div><p>(A–C) The posterior part of XX E7.5 <i>X^GFP</i> embryos, which inherited X-linked GFP either from the mother (A) or the father (B), were cultured for 48 hours and immunostained for Oct4 to identify the PGCs. White arrows depict PGCs that do or do not co-express GFP. The expression of GFP was analysed in the total number of PGCs per embryo (C). Green bars depict the median, <i>n</i> is the total number of embryos analysed.</p><p>(D,E) The posterior part of XX E7.5 <i>Blimp1:gfp</i> embryos were cultured for 48 hours and immunostained for H3K27me3. The total number of Blimp1(GFP)-positive PGCs was screened for the presence of a H3K27me3-positive nuclear accumulation</p><p>(D) Red bar depicts the median, <i>n</i> is the total number of embryos analysed. A representative image of the posterior cluster containing the Blimp1(GFP)-positive PGCs after 48 hours culture is shown in (E). White arrows show Blimp1(GFP)-positive PGCs that contain a prominent H3K27me3 nuclear accumulation.</p>