ECTV Infection Induces Increased Expression of NKG2D Ligands In Vitro and In Vivo

<div><p>(A) MEFs were infected with 0.5 pfu ECTV 189898-p7.5-EGFP for 18 h. Cells were analyzed for staining with the indicated reagents after gating for EGFP⁻ cells (uninfected) and EGFP⁺ cells (infected). Data correspond to one typical experiment from three similar experiments. Shaded area, infected cells stained with isotype-matched control Ig or secondary Ab alone; black line, infected cells stained with the indicated reagent; gray line, non-infected cells stained with the indicated reagent.</p><p>(B) qRT-PCR was performed as described. Data were normalized to the amount of β-actin mRNA. Data are representative of two similar experiments.</p>