The Effect of B14 on IKKα and IKKβ-Induced NF-κB Activation

<p>HEK 293 cells were co-transfected with reporter plasmids for NF-κB (90 ng), TK-RhL as internal control (10 ng), and the indicated amount of plasmids expressing either wild type IKKα or IKKβ (A), constitutively active IKKα or IKKβ (B), or B14 (A and B). The total amount of DNA applied in each reaction was adjusted to 500 ng using pCI empty vector. After 24 h, the cells were lysed and the activities of both luciferases were measured using Dual-specific luciferase assay kit (Promega) in triplicates. Differences in the fold activation of the NF-κB reporter induced by the indicated IKK subunit in cells transfected with the empty vector and cells expressing B14 were analysed by Student's <i>t</i>-test and the <i>p-</i>values are indicated: * <i>p</i> < 0.05 or ** <i>p</i> < 0.01.