Loss of CCR5⁺ and Effector Memory CD4⁺ T Cells from GALT during HIV-1 Infection

<div><p>(A) Comparison of the levels of CD4⁺ or CD8⁺ human T cells in the GALT of representative naive and HIV-1–infected mice.</p> <p>(B) Box plot depicting the levels of GALT CD4⁺ T cells for naive (green) and HIV-1–infected (white) BLT mice.</p> <p>(C) Comparison of the levels of human CD4⁺CCR5⁺ T cells in the GALT of naive and HIV-1–infected BLT mice. Significantly fewer GALT CD4⁺ T cells had detectable CCR5 expression levels following HIV-1 infection.</p> <p>(D) Box plot depicting the levels of CD4⁺CCR5⁺ T cells in the GALT for naive (green) and HIV-1–infected (white) BLT mice.</p> <p>(E and F) Comparison of the levels of human CD4⁺ effector memory T cells in the small intestine intra-epithelial (E) and lamina propria (F) lymphocyte compartments of representative naive and HIV-1–infected BLT mice. HIV-1–infected BLT mice have statistically fewer effector memory CD4⁺ T cells present in their small intestines.</p> <p>(G) Box plot depicting the levels of CD45RA^negCD27^neg effector memory T cells in the small intestine of naive (green) and HIV-1–infected (white) BLT mice. In the box plots, the boxes extend from the first to the third quartiles, enclosing the middle 50% of the data. The middle line within each box indicates the median of the data, whereas the vertical line extends from lowest to the highest values. Naive (<i>n</i> = 4), HIV-1 infected (<i>n</i> = 4). Gating strategy for flow cytometric analysis: live cells → human CD45 → human CD3 → human CD4 → CCR5, CD27, or CD45RA.</p> <p>IEL, intraepithelial lymphocytes; LI large intestine; LPL, lamina propria lymphocytes; SI, small intestine.</p>