<i>E. coli</i> K-12 strains and plasmids

<p>Abbreviations: Amp^R, ampicillin resistant; Kan^R, kanamycin resistant; Cam^R, chloramphenicol resistant; Temp^S, temperature sensitive for growth. The Δ<i>rap514</i> and Δ<i>orf432</i> alleles are deletions of λ base pairs 42,440–43,037 and 40,621–41,073, respectively.</p><p>Footnotes: ¹ Other genetic elements present in 594: <i>galK2</i>(<i>Oc</i>), <i>galT22</i>, <i>lac-3350</i>, <i>rpsL179</i>, <i>IN</i>(<i>rrnD-rrnE</i>)<i>1</i>.</p>2<p>Other genetic elements present in the C600 strain background: <i>supE</i>, <i>thi-1</i>, <i>thr-1</i>, <i>leuB6</i>, <i>lacY1</i>.</p>3<p>Other genetic elements present in JC11450: <i>thr-1</i>, <i>leuB6</i>, Δ(<i>gpt-proA</i>)<i>62</i>, <i>hisG4</i>, <i>argE3</i>, <i>thi-1</i>, <i>ara-14</i>, <i>lacY1</i>, <i>galK2</i>, <i>xyl-5</i>, <i>mtl-1</i>, <i>tsx-33</i>, <i>rpsL31</i>, <i>kdgK51</i>.