Anti-saliva antibody response following immunization of BALB/c mice with <i>Lutzomyia intermedia</i> SGS.

<p>BALB/c mice (3–5 per group) received 3 inoculations of PBS (open bars) or SGS (closed bars). (A) ELISA was performed with sera from mice inoculated with PBS (open bars) or SGS (closed bars). (B) IgG subclasses present in immune sera were determined by ELISA using IgG1, IgG2a and IgG2b conjugates. Purified IgG1, IgG2a and IgG2b (dotted bars) were employed as control IgG subclass (positive controls). Bars represent the means and standard errors of the means from three independent experiments (*p<0.05). (C) Commassie blue-stained SDS-PAGE gel after electrophoresis of <i>L. intermedia</i> (L.int) and <i>L. longipalpis</i> (L.lon) SGS. The numbers represent the position of the mol wt markers. (D) Western blots of the gels showed in C, probed with sera from <i>L. intermedia</i> SGS immunized mice.