Na,K-ATPase function is impaired in rat lungs exposed to hypercapnic acidosis.

<p>(A) Basolateral membranes (BLM) were purified from the peripheral lung tissue of rat lungs exposed to 40 mmHg CO₂(pH_e: 7.4) or 60 mmHg CO₂(pH_e: 7.2), and Na,K-ATPase activity was measured as [γ-³²P]ATP hydrolysis. Graph represents the mean±SEM, (n = 3). (B) BLM and total membranes were purified from the peripheral lung tissue of rat lungs treated as (A), and Na,K-ATPase protein abundance was assessed by Western blot. Graph represents the mean±SEM, (n = 3). Representative blots of Na,K-ATPase α₁-subunit at the BLM and total membrane protein abundance are shown. pH_e: extracellular pH. * p<0.05.