Selective Inhibition of HIV-1 RNA Splicing <i>Ex Vivo</i> by IDC16

<div><p>(A) Drawing and formula of IDC16 compound.</p><p>(B) Schematic representation of HIV-1 genome. The 5′ splice sites (D1–D4) and 3′ splice sites (A1–A7) are indicated. The various open reading frames are boxed.</p><p>(C) HeLa cells transfected with the pΔPSP construct were either untreated (lane 7) or treated with 0.05 μM, 0.1 μM, 0.5 μM, 1 μM, 2.5 μM, or 5 μM of compound IDC16 (lanes 1–6, respectively). Multiply spliced products of HIV-1 RNA were amplified by RT-PCR using the oligonucleotide primers BSS and SJ4.7A. The PCR products were analyzed by polyacrylamide gel electrophoresis after normalization with <i>GAPDH</i> (see <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.0030159#s4" target="_blank">Materials and Methods</a>). Nomenclature of the RT-PCR products on the left of the panel is according to [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.0030159#ppat-0030159-b030" target="_blank">30</a>]. Size markers (in bp) are shown on the right of the panel (lane 9). RT-PCR from untransfected HeLa cells (lane 8).</p>