H3.3-FLAG Is Deposited in the Germ Line Chromatin in Blastoderm Embryos

<div><p>Confocal sections of blastoderm embryos stained for DNA (blue), H3.3-FLAG (green), and H3K4me3 (red).</p><p>(A) H3.3-FLAG (left inset) is deposited at equivalent levels in somatic (arrows) and germ line (arrowheads) nuclei in wild-type embryos. H3K4me3 is enriched in somatic nuclei (right inset).</p><p>(B) An identical situation is observed in <i>Hira^HR1</i> embryos. Bar: 10 μm.</p>