<p>Mitochondrial complex I activity was taken as the one that is rotenone sensitive. Specific enzyme activities shown as nmol/min/mg protein. I; NADH-DB oxidoreductase, II; Succinate-DB oxidoreductase, II+III; Succinate Cytochrome C reductase, IV; Cytochrome C oxidase, CS; Citrate synthase. ** and * indicate <i>P</i> values that are stastically significant (p<0. 05 and p<0.005). SD± was calculated for only five cell lines.</p
<p>Enzyme activities are expressed as</p><p>*cU/U citrate synthase (CS) and</p><p>**nmol.min<sup>−1<...
The mitochondrial toxin rotenone exerts cytotoxicity via overproduction of reactive oxygen species (...
<p>A,B. Oxygen consumption was assessed by high-resolution respirometry in digitonin-permeabilized N...
<p><b>A.</b> Western blot analysis of a representative subunit from each complex. Ponceau S staining...
<p>Cells were cultured and then mitochondrial fractions were isolated by centrifugal fractionation. ...
<p>Cells were cultured in the presence of 50 and 100 µM rotenone for 6 hr and the mitochondrial frac...
Complex I activity of the mitochondrial respiratory chain is difficult to measure in blood lymphocyt...
Contains fulltext : 51699.pdf (publisher's version ) (Closed access)Complex I acti...
<p>Enzyme activity of mitochondrial citrate synthase, respiratory complex I and IV was measured spec...
<p>(A) NADH: coenzyme Q oxidoreductase (Complex I); (B) Succinate: coenzyme Q oxidoreductase (Comple...
<p>Ratio of ADP-stimulated (S3) and baseline oxygen consumption (S4) in MPC and MTT. <b>B</b> Baseli...
We have developed a flow cytometric assay to measure the oxidative capacity of cultured lymphoblasts...
The assessment of mitochondrial respiratory chain (RC) enzymatic activities is essential for investi...
<p>Enzymatic activities of Complex I and II were measured in total cell lysates using spectrophotome...
The assessment of mitochondrial respiratory chain (RC) enzymatic activities is essential for investi...
<p>Enzyme activities are expressed as</p><p>*cU/U citrate synthase (CS) and</p><p>**nmol.min<sup>−1<...
The mitochondrial toxin rotenone exerts cytotoxicity via overproduction of reactive oxygen species (...
<p>A,B. Oxygen consumption was assessed by high-resolution respirometry in digitonin-permeabilized N...
<p><b>A.</b> Western blot analysis of a representative subunit from each complex. Ponceau S staining...
<p>Cells were cultured and then mitochondrial fractions were isolated by centrifugal fractionation. ...
<p>Cells were cultured in the presence of 50 and 100 µM rotenone for 6 hr and the mitochondrial frac...
Complex I activity of the mitochondrial respiratory chain is difficult to measure in blood lymphocyt...
Contains fulltext : 51699.pdf (publisher's version ) (Closed access)Complex I acti...
<p>Enzyme activity of mitochondrial citrate synthase, respiratory complex I and IV was measured spec...
<p>(A) NADH: coenzyme Q oxidoreductase (Complex I); (B) Succinate: coenzyme Q oxidoreductase (Comple...
<p>Ratio of ADP-stimulated (S3) and baseline oxygen consumption (S4) in MPC and MTT. <b>B</b> Baseli...
We have developed a flow cytometric assay to measure the oxidative capacity of cultured lymphoblasts...
The assessment of mitochondrial respiratory chain (RC) enzymatic activities is essential for investi...
<p>Enzymatic activities of Complex I and II were measured in total cell lysates using spectrophotome...
The assessment of mitochondrial respiratory chain (RC) enzymatic activities is essential for investi...
<p>Enzyme activities are expressed as</p><p>*cU/U citrate synthase (CS) and</p><p>**nmol.min<sup>−1<...
The mitochondrial toxin rotenone exerts cytotoxicity via overproduction of reactive oxygen species (...
<p>A,B. Oxygen consumption was assessed by high-resolution respirometry in digitonin-permeabilized N...