Expression of ENPP1 decreases in the early maturation phase of 3T3-L1 into adipocytes.

<p>3T3-L1 cells were induced to differentiate into adipocytes by desamethasone, 3-iso-butyl-1-methylxanthine and insulin (DMI). The RNA was isolated at different time points, as indicated. Data show real time PCR for expression of ENPP1 and other genes involved in adipocyte maturation. Mean and SD from 3 experiments are presented in arbitrary units after correction for 18S expression. One-way ANOVA p-value for multiple group comparison was <0.05 for each of the gene expression data shown in figure. Pre-adipocyte are shown as dark bars, mature adipocyte are shown as open bars