ΔRS N-terminus mutations that improve splicing also increase the ability of ΔRS to bind IgM M1-M2.

Stephanie D. Shaw (378658)
Sutapa Chakrabarti (378659)
Gourisankar Ghosh (230073)
Adrian R. Krainer (31173)

Publication date

February 2013

DOI

10.1371/journal.pone.0000854.g003

Abstract

<p>(A) Recombinant SF2/ASF and mutant proteins employed in the crosslinking assay. M: molecular-weight markers. (B) UV crosslinking of SF2/ASF and variant proteins to radiolabeled IgM M1-M2 RNA. BSA (lane 1), or purified recombinant SR proteins SF2/ASF (lane 2), ΔNSF2/ASF (lane 3), ΔRS (lane 4), ΔNΔRS (lane 5), ΔRS: Δ5-10 (lane 6), and ΔRS:R8E (lane 7) were incubated with uncapped IgM M1-M2 RNA prior to crosslinking, RNAse digestion, and separation of crosslinked adducts by SDS-PAGE.</p

Extracted data

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ΔRS N-terminus mutations that improve splicing also increase the ability of ΔRS to bind IgM M1-M2.

Abstract

Extracted data

ΔRS N-terminus mutations that improve splicing also increase the ability of ΔRS to bind IgM M1-M2.

Abstract

Extracted data

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