Fluorescent photographs of unfixed RBs and EBs of <i>C. trachomatis</i> serovar L2.

<p>RBs (A, B, and C) and EBs (D, E, and F) were harvested and purified at 24 and 48 h p.i. respectively, and probed with pAb against fragment 2 of PmpD (A and D), anti-MOMP pAb (B and E), and rabbit pre-immune sera (C and F). Staining of EBs with anti-PmpD pAb was negligible while anti-MOMP Pab stained both RBs and EBs indicating that the PmpD passenger domain may be secreted during the conversion of RBs into EBs. The Figure was scanned using Image Pro-Plus 4.1 and prepared with Adobe Photoshop 6.0.