Reactivation of PFV-Infected Resting Cells

<div><p>(A) Sub-cellular localization of Gag proteins (green) studied by confocal microscopy following indirect immunofluorescence using anti-Gag antiserum. Thirty days p.i., Gag proteins mainly localize at the MTOC (stained with anti-γ-tubulin abs in red) in resting cells. In contrast, Gag proteins are detected both in the cytoplasm and the nucleus of these cells at 24, 48, and 96 h post-activation by splitting and serum addition. Syncitia, characteristic of a productive FV infection, appear 96 h post-reactivation.</p><p>(B) Western blot analysis of protein extracts from resting and activated PFV-infected cells with or without CHX treatment (150 μg/ml). At 30 d p.i. (with m.o.i. of 2), only the Gag doublet at 68 and 71 kDa is detected in resting cells using anti-Gag abs. Under CHX treatment, at 24 h post-reactivation, Gag cleavages are clearly detected, including the 38-kDa Gag product (*) involved in virus uncoating [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.0030074#ppat-0030074-b020" target="_blank">20</a>].</p>