Intracellular Localization of Incoming PFV in Quiescent and Activated CD4-positive T Cells

<div><p>(A) Flow cytometry profile of isolated human resting CD4-positive T cells. Purity of sorted cells is at least 99% and no cell activation is observed 5 d p.i.</p><p>(B) Sub-cellular localization of Gag proteins studied by confocal microscopy following indirect immunofluorescence using anti-Gag antiserum (green). In activated CD4-positive T cells at 48 h p.i., Gag proteins are not restricted to the MTOC but diffusely localize in the cytoplasm and in the nucleus (top row). In resting CD4-positive T cells at 2 and 5 d p.i., Gag proteins co-localize with γ-tubulin (red), the MTOC marker. Twenty-four hours following cell stimulation of 5 d−infected resting CD4-positive T cells, Gag proteins diffusely localize in the cytoplasm and in the nucleus, similar to what we observed in activated CD4-positive T cells (bottom row).</p>