Nascent and Mature Transcripts Visualized by In Situ Hybridisation

<p>Fluorescent staining by ISH for <i>her1</i> (top left)<i>, her7</i> (top middle)<i>,</i> and <i>deltaC</i> (top right)<i>,</i> using tyramide chemistry (green), reveals nuclear dots corresponding to nascent transcripts and cytoplasmic signal corresponding to mature mRNA. The bottom panel is a magnified detail of the top left panel. The images are confocal optical sections of flat-mounted specimens, counterstained for DNA with TOPRO3 (blue false colour). Red staining shows <i>myoD</i> expression by dual ISH.