GAAPs Are Evolutionarily Conserved

<div><p>(A) CLUSTALW [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.0030017#ppat-0030017-b047" target="_blank">47</a>] alignment of h-GAAP and v-GAAP with related proteins from other species: VACV strain Evans, CMLV strain CM-S, human, orangutan, dog, mouse, <i>X. laevis,</i> zebrafish, <i>A. thaliana,</i> and D. melanogaster. Identical residues are shown in black, and residues conserved in six to eight or nine out of ten sequences are in light or dark grey, respectively. Horizontal bars show predicted transmembrane domains (I–VII) based on the Kyte–Doolittle hydropathy model. An asterisk denotes a block of 20 aa.</p><p>(B) Hydrophobicity profiles of human, VACV, and A. thaliana GAAPs according to Kyte and Doolittle, plotted using the program WinPep [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.0030017#ppat-0030017-b048" target="_blank">48</a>]. Putative transmembrane domains (I–VII) are outlined.</p>