A Network Gauge Predicts Cell Behavior and Suggests Critical Elements of Network Architecture

<p>A nine-signal PLSR model trained on parental data predicts migration and proliferation in 24H cells. The line thickness emerging from each protein indicates the relative average importance of each protein in the model for migration (black) or proliferation (gray). Prediction of migration or proliferation is a function of model importance and the amount of phosphorylation measured in either parental cells or 24H cells. The proteins constituting the model are transferrin receptor (TfR), annexin A2 (An A2), solute carrier protein 38 (SCF38), SH2-containing protein (Shc), SH2-containing inositol polyphosphate 5-phosphatase (SHIP-2), and activated cdc42-associated kinase 2 (ACK2). Previously documented associations with endocytosis (red circle), PIP3/PI3K signaling (blue circle), or both (yellow circle) are shown. The absence of association with either endocytosis or PIP3/PI3K signaling is denoted by a white circle (An A2).