Image Processing and 3D Reconstructions of RECQ1 Oligomers

<div><p>Representative images from the various stages of image-processing procedures using negatively stained untagged RECQ1 + ssDNA sample as an example.</p> <p>(A) Examples of individual particle images after band-pass filtering and alignment. These images are members of the corresponding class averages shown in the row below (protein is white).</p> <p>(B) Class averages obtained after classification.</p> <p>(C) Re-projections of the 3D density map in orientations corresponding to the Euler angles assigned to the class averages in the row above.</p> <p>(D) Surface renderings of the density map at a threshold level of σ = 3 in orientations corresponding to the Euler angles assigned to the class averages in (B). The left- and right-most images correspond to approximately diametrically opposite views of the molecule, whereas the images in-between correspond to views that are rotated by approximately 90° with respect to these, and also with respect to each other. Scale bar represents 10 nm.</p>