Development of anticipatory increase in locomotor activity prior to food administration in wildtype (A) and ACHE mutants (B) of different age, and in IR zebrafish (C).

<div><p>X-axis: % change in activity level within 30 min prior to food administration, as compared to preceding 90-min period.</p> <p>First day of significant (p<.05) change in group behavior in young (*) and middle-aged (**) fish.</p> <p>Note that BL was assessed after 7 days of new timing of daily food administration in white/white environment.</p> <p>Young fish – diamond, middle-aged fish – square, old fish – triangle.</p> <p>Wildtype – black; ACHE mutants – green; IR zebrafish – orange.</p>