Inhibition of NF-κB Signaling Does Not Impair Lytic Replication In Vitro or Acute Replication in the Lungs

<div><p>(A) Single-step growth curve in MEFs, with an MOI of 5.0 PFU per cell with the indicated virus. Samples were harvested at the indicated time points and titers were determined on NIH 3T12 fibroblasts as described in Materials and Methods.</p><p>(B) Multistep growth curve in MEFs, with an MOI of 0.05 PFU per cell with the indicated viruses.</p><p>(C) Multistep growth curve in NIH 3T12 cells, with an MOI of 0.05 PFU per cell with the indicated viruses. Data are representative of at least two independent experiments.</p><p>(D and E) C57Bl6 mice were infected with 1,000 PFU by the intranasal route of inoculation with the indicated viruses. On the indicated days postinfection, lungs (D) and spleens (E) were harvested, disrupted, and titered on NIH 3T12 cells. The data were compiled from one or two experiments with three to five mice analyzed per experiment. Data are shown as log10 titer, and the bar indicates the geometric mean titer. The dashed line indicates the limit of detection of this assay as log101.7 or 50 PFU/ml of sample homogenate.</p>