EGFP localization in ocular tissues following intravitreal injection.

<div><p>Direct fluorescence microscopy was utilized to localize EGFP expression in ocular tissues at 2-days post-injection of 0.6 µg of compacted DNA.</p> <p>(<b>A</b>) Retinal autofluorescence was present in the outer segment of all samples including mock- and naked plasmid-injected eyes, however EGFP fluorescence was detected in the GCL of both the AC-GFP and TFA-GFP injected eyes.</p> <p>(<b>B</b>) Upon examination at higher magnification, EGFP expression was confined to the GCL in the AC-GFP-injected eyes, and present in the GCL and IPL of TFA-GFP-injected eyes.</p> <p>(<b>C</b>) Expression of EGFP was also detected in the cornea, trabecular meshwork, and lens following intravitreal injection of both TFA and AC nanoparticles (only AC shown).</p> <p>“FITC” channel imaging was performed as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0000038#s2" target="_blank">Methods Section</a>.</p> <p>OS: Outer Segment; ONL: Outer Nuclear Layer; INL: Inner Nuclear Layer; IPL: Inner Plexiform Layer; GCL: Ganglion Cell Layer; CO: Cornea; TM: Trabecular Meshwork; LE: Lens.</p> <p>Scale bars, 10 µM.</p>