Ocular expression levels of EGFP mRNA after subretinal injection.

<div><p>At 2-days post-subretinal injection of 0.6 µg of compacted DNA, the lens, PECS, and retina were isolated, and qRT-PCR was used to assess EGFP mRNA levels.</p> <p>Both the AC-GFP and TFA-GFP nanoparticles produced substantial expression in the PECS and retina.</p> <p>In both tissues, the AC-GFP nanoparticle generated EGFP mRNA levels nearly 2-fold higher than the TFA-GFP nanoparticle.</p> <p>EGFP expression was also observed in the lens; however there was significant deviation between samples, possibly reflecting the amount of nanoparticle reflux into the vitreous because of back-pressure during syringe withdrawal following some of the subretinal injection procedures.</p>