Activated B Cells Are Decreased in the Lungs and Spleens of IκBαM-Infected Mice

<div><p>Cells were prepared from the lungs and spleens harvested from four uninfected naïve mice or from four or five mice that were infected 16 and 42 d prior to harvest with 1,000 PFU of WT γHV68 or IκBαM.1. Cells from individual mice were surface stained with anti-CD19 conjugated to allophycocyanin and anti-CD69 conjugated to FITC and analyzed by flow cytometry.</p><p>(A) Flow cytometric dotplots of stained cells from the lungs (upper panel) and spleens (lower panel). Values shown in the upper right quadrant of each dotplot are the percentages of CD19⁺ cells that express the CD69 activation marker in a representative experimental sample.</p><p>(B) Each point in the scatterplot represents the percentage of CD19⁺CD69^hi cells from the lung of a single mouse; the bar represents the mean percentage. The percentage of CD19⁺CD69^hi cells in the lungs of mice infected with IκBαM.1 was significantly different from that of mice infected with WT γHV68; <i>p</i> = 0.0007 at 16 dpi.</p><p>(C) Each point in the scatterplot represents the percentage of CD19⁺CD69^hi cells from the spleen of a single mouse; the bar represents the mean percentage. The percentage of CD19⁺CD69^hi cells in the spleens of mice infected with IκBαM.1 was significantly different from that of mice infected with WT γHV68; <i>p</i> < 0.0001 at 16 dpi and <i>p</i> = 0.0042 at 42 dpi.</p>