Figure 2

<div><p> <i>cno²</i> zygotic null mutants display gain and loss of progenitors.</p> <p>All panels show lateral views of stage 11 embryos stained with an α-Eve antibody.</p> <p>(A) Eve wildtype (wt) expression in a subset of dorsal muscle/heart progenitors and founder cells (2–3 cells) per hemisegment.</p> <p>(B) <i>cno²</i> mutants show gain (arrow), loss (arrowhead) or a wildtype number (asterisk) of Eve⁺ progenitors in different hemisegments.</p> <p>(C) Embryos in which N^Act, Wg and Ras^Act are simultaneously expressed in the mesoderm display a <i>cno²</i>-like phenotype: gain (arrow), loss (arrowhead) and wildtype number of Eve⁺ progenitors (asterisk).</p> <p>(D) Overexpression of Ras1^Act in the mesoderm induces the specification of extra Eve⁺ progenitors.</p> <p>(E) Overexpression of N^Act in the mesoderm inhibits Eve⁺ progenitor specification.</p> <p>(F) Embryos in which Wg is overexpressed in the mesoderm show gain of Eve⁺ progenitors in intersegmental regions (arrow).</p> <p>(G) Table shows % of embryos of the indicated genotype that display a wildtype number of Eve⁺ progenitors, Eve⁺ progenitors gain, loss and both (gain and loss).</p> <p> <i>cno²</i> and <i>cno^mis1</i> are null and hypomorph mutant alleles, respectively; <i>Df(3R)^6-7</i> removes <i>cno</i>. <i>cno</i> gof phenotype (<i>UAS-cno</i>) is also variable.</p> <p>Legends for bars are shown below the table.</p> <p>At least 70 embryos of each genotype were counted. P<0.0001 for all genotypes except for <i>cno^mis1</i> (P = 0.0017).</p>