Cir1 Directly Regulates Genes Required for Iron Transport

<div><p>(A) Cluster analysis of genes required for iron transport show two patterns of differential expression in the <i>cir1</i> mutant (serotype D), resulting in two main clusters (I and II). Columns represent the log-transformed ratio of the array data from the wild-type strain in low- versus high-iron medium (WT LIM vs. LIM + Fe), the <i>cir1</i> mutant versus the wild-type strain in low-iron medium (MT vs. WT LIM), the <i>cir1</i> mutant versus the wild-type strain in high-iron medium (MT vs. WT LIM + Fe), and the <i>cir1</i> mutant in low- versus high-iron medium (MT LIM vs. LIM + Fe).</p> <p>(B) RNA blot analysis to confirm differential expression of genes required for iron transport in both the serotype D and the serotype A strains grown in a low-iron medium (L) or high-iron medium (H). WT, wild type.</p>