Defining Targets of Antibodies on DBL3X

<p>Models of DBL3X in which the intensity of the gray and blue indicates reactivity of plasma tested on a peptide array (PepScan scores). Gray indicates lowest scores and dark blue indicates highest scores. (A and C–I) represent reactivity in eight individual plasma samples. (B) shows the reactivity in the same plasma as tested in (A), but the model has been rotated 180 degrees. Arrow 1 indicates a highly recognized conserved α-helix region, which was not predicted to be an epitope by BepiPred and was characterized by low dN/dS ratios and low sequence variation. Arrow 2 indicates another α-helix with a predicted B cell epitope containing some residues with high sequence variation. Variable regions V1 and V2 are fairly well recognized by most of the serum samples. GB indicates the glycan-binding site of EBA-175.