COPI-Dependent Golgi Disassembly in DCV Infected Cells

<div><p>Confocal analysis of cells pretreated with the indicated dsRNA and infected with DCV.</p><p>(A) Golgi morphology of DCV-infected control cells (GFP) reveals that the normal punctate staining in uninfected cells is dispersed during viral replication.</p><p>(B–F) Loss of COPI <i>(bCOP)</i> (B), <i>SREBP</i> (E), or <i>CG3523</i> (F) but not COPII <i>(sec23)</i> (C) or <i>Syx5</i> (D) results in a decrease in viral infection. Note that the Golgi stain is reduced in uninfected COPI, COPII, <i>SREBP, CG2523,</i> and <i>Syx5,</i> but only the loss in COPI, <i>SREBP,</i> or <i>CG3523</i> results in a decrease in DCV replication. Green, anti-Golgi <i>(DG13);</i> red, anti-DCV; blue, Hoescht 33342.</p>