The Effects of Relative Promoter Strength and N Gene Rearrangement on the Growth Phenotype of VSV

<div><p>(A) The balance of the production between genome and anti-genome is determined by the promoter strength of the anti-genome relative to that of the genome (<i>S_prom</i>). The changes of virion production in BHK and DBT cells by the variation of the parameter are observed. The black circles indicate the virion productions of wild-type (<i>S_prom</i> = 5.4) in BHK and DBT cells.</p><p>(B) The extension of phenotypic variations by double genomic manipulations was predicted. As an important phenotype of virus for vaccine use, the changes of virion production by the relocation of N gene along with the variation in the promoter strength of the anti-genome relative to that of the genome (<i>S_prom</i>) are shown. The black circle indicates the virion production of wild-type in BHK cells. V_ex(0) = 3.</p>