<i>svb</i> Target Genes Are Involved in Separate Features of Denticle Edification

<div><p>(A) Subcellular localization of Singed, Forked, and Miniature in the epidermis of stage 15 wild-type embryos. Distribution of α-catenin, a component of adherens junctions that underlines the cell contour, was observed in embryos expressing α-catenin-GFP driven by E22C-Gal4. Red indicates F-actin, and green indicates α-catenin, Singed, Forked, and Miniature.</p> <p>(B) Transmission electron microscopy analysis of denticle cells from wild-type <i>(wt)</i>, <i>m¹,</i> and <i>sn³, f^36a</i> double mutant embryos. As in smooth cells, the flat region of the apical cell face organizes microvilli that contact cuticle layers only at the apex. In contrast, the plasma membrane is in close contact with cuticle along the entire wild-type denticle contour. Although the <i>m¹</i> mutation alters this membrane/cuticle contact, no defects are visible in <i>sn³, f^36a</i> embryos. Close-up pictures show a region of the growing extension. Scale bar represents 0.25 μm.</p>