Cisplatin treatment inhibits the autophagic response in A375 cells.

<p>A375 melanoma cells were treated for 24 h with single or combined compounds: 60 mM trehalose (Tre), 20 µM cisplatin (Cis), 50 µM ac-DEVD-CHO (CHO); Cis A: cisplatin-treated adhering cells; Cis F: floating cells. Western blot of Beclin-1 and densitometric analysis of a typical experiment out of 3 are reported. *In order to better visualize the Beclin-1 proteolytic fragment, the photographic film was over-exposed. (<b>B</b>) Western blot of Atg14; a typical experiment out of 3 is reported. *In order to better visualize the full-length Atg14 recovery by ac-DEVD-CHO, the photographic film was over-exposed. (<b>C</b>) Western blot of LC3 and densitometric analysis of a typical experiment out of 3 are reported. Densitometric analyses were normalized to Ponceau S staining.