Enhanced ionomycin induced eryptosis and adhesion of erythrocytes from annexin7-deficient mice. A.

<p>Histogram of annexin V-binding reflecting phosphatidylserine exposure in a representative experiment of erythrocytes from annexin7-deficient mice (<i>anx7^−/−</i>) and their wild type control mice (<i>anx7^+/+</i>) exposed for 30 min to Ca²⁺ ionophore ionomycin (1 µM). <b>B.</b> Arithmetic means ± SEM (n = 8−9) of the percentage of annexin V-binding erythrocytes from annexin7-deficient mice (<i>anx7^−/−</i>, black bars) and their wild type control mice (<i>anx7^+/+</i>, white bars) exposed for 30 min to Ringer without (left bars) or with (right bars) ionomycin (1 µM). ### significant (p<0.001) difference from absence of ionomycin, *** significant difference (p<0.001) from <i>anx7^+/+</i> erythrocytes (ANOVA). <b>C.</b> Arithmetic means ± SEM (n = 8−9) of the forward scatter of erythrocytes from annexin7-deficient mice (<i>anx7^−/−</i>, black bars) and their wild type control mice (<i>anx7^+/+</i>, white bars) exposed for 30 min to Ringer without (left bars) or with (right bars) ionomycin (1 µM). ### significant (p<0.001) difference from absence of ionomycin, *** significant difference (p<0.001) from <i>anx7^+/+</i> erythrocytes (ANOVA). <b>D.</b> Arithmetic means ± SEM (n = 6) of the number of erythrocytes from annexin7-deficient mice (<i>anx7^−/−</i>, black bars) and their wild type control mice (<i>anx7^+/+</i>, white bars) adhering to the human umbilical vein endothelial cells (HUVEC) following exposure for 30 min to Ringer without (left bars) or with (right bars) ionomycin (1 µM). ### significant difference (p<0.001) from absence of ionomycin, *** significant difference (p<0.001) from <i>anx7^+/+</i> erythrocytes (ANOVA).