Inhibition of the lysosomal protease decreased the apoptosis induced by EV71.

<p>(A) When RD-A cells infected with EV71 or without EV71 were co-cultured with different dosage of E64d and pepstatin A for 12 hours, the cells were lyzed and Western blot were performed. The results of western are representatives of the three independent experiments. The statistical analysis of PARP/Actin and caspase-3/Actin are the mean of three independent experiments. Mock value is set on 100% each experiment. (B) RD-A cells treated as (A) were collected and stained with Annexin V-FITC and PI. The flow cytometry analysis was performed. The percentage of apoptotic cells were shown in the form of histogram. Data are shown as the mean of three independent experiments.