Basal and insulin-stimulated glucose uptake in cultured skeletal myotubes.

<p>Conditioned media (2% in final media volume) from <b>(a,</b> basal state; <b>b</b> insulin-stimulated<b>)</b> primary human and <b>(c,</b> basal state; <b>d</b> insulin-stimulated<b>)</b> THP-1 macrophages treated with vehicle (PBS), 75 µg/ml acLDL/10 µg/ml Sandoz compound with or without 50 µg/ml HDL for 18 hours was placed on human primary skeletal myotubes for 24 hours. Cells were treated with insulin (100 nM) or vehicle for 30 minutes before glucose uptake was measured. n = 6/group; data are presented as mean ± SEM; *indicates significantly different from Con and acLDL+HDL, P<0.05.