Stimulation of IL-32 by Mycobacteria

<div><p>(A) Whole blood was diluted 1:4 with RPMI and stimulated with 10 μg/ml sonicated M. tuberculosis (MTB) or M. bovis BCG (BCG). Triton X-100 (0.5%) was added 24 h later, and IL-32 concentration was measured by ECL.</p> <p>(B) Stimulation of freshly isolated PBMCs with various concentrations of LPS or sonicated M. tuberculosis stimulated IL-32 synthesis in a dose-dependent manner.</p> <p>(C and D) Freshly isolated PBMCs were stimulated with TLR4 (LPS, 100 ng/ml), TLR2 (Pam3Cys, 10 μg/ml), TLR3 (poly I:C, 5 μg/ml), M. tuberculosis, M. bovis BCG, or S. aureus (all at 1 × 10⁷ microorganisms/milliliter, heat-killed). After 24 h of stimulation, LPS-, <i>M. tuberculosis–,</i> and M. bovis BCG–stimulated production of IL-32 (C) or IL-6 (D) was measured by ECL.</p> <p>Data are presented as means ± SEM (<i>n =</i> 8).</p>