pdgf-b Expression Is Upregulated in Regenerating Zebrafish Hearts

<div><p>(A) RT-PCR analysis of <i>pdgf-b</i> and <i>pdgfr-</i>α. β <i>-actin</i> was used as a loading control. Expression of <i>pdgf-b</i> began to increase at 3 dpa and lasted until 14 dpa during zebrafish heart regeneration. <i>pdgfr-</i>α was expressed in both sham-operated and regenerating hearts; its expression level does not change. </p> <p>(B) In situ hybridization using a radioactive antisense probe showing <i>pdgf-b</i> expression in sham-operated and regenerating hearts at 7 dpa. Expression <i>of pdgf-b</i> was localized to the wound site. A radioactive sense probe was used as a negative control. The dashed red line marks the amputation plane. </p>