Expression of IFN-α2 and IFN-α8 by Immature DCs and Monocytes Cultured from an APS1 Patient Relative to Two Healthy Blood Donors

<p>Expression by immature DCs (A) and monocytes (B). The APS1 donor has typical clinical features of APS1 and high anti–type I IFN titres (see footnote of <a href="http://www.plosmedicine.org/article/info:doi/10.1371/journal.pmed.0030289#pmed-0030289-t001" target="_blank">Table 1</a>). RT-PCR reactions were performed in parallel with <i>HPRT</i> as a “house-keeping” gene; <i>GADPH</i> gave very similar results when tested in one of the healthy donors, C1 (for IFN-α8). The relative gene (mRNA) expression levels were calculated using the comparative Ct (ΔΔCt) method (according to Applied Biosystems) to yield 2^−ΔΔCt, where Ct represents the threshold cycle. Every sample was run at least three times in three parallel reactions. Results are depicted in histogram format as ratios of the expression levels in cells from the APS1 donor (which have been given a nominal value of 1.0) to those in the cells from the controls, C1 and C2. The bars represent the standard error of the mean of each ratio.