Effect of <i>Eif1</i> Upregulation on <i>Xist</i> mRNA Levels

<div><p>Quantification of <i>Eif1</i> (A) and <i>Xist</i> (B) RNAs in undifferentiated parental female ES cell line (LF2), clones knocked down for <i>Eif1,</i> and clones lipofected with the pTriEx-Eif1 expression plasmid (see Methods and Materials) after 4 d of selection on G418-containing medium.</p><p>Columns and bars show the mean ± SD (n = 3), expressed in arbitrary units, of the quantity of <i>Eif1</i> and <i>Xist</i> transcripts standardized over <i>Rrm2</i> RNA.</p>