Effect of Murine Type I IFNs on Recombinant Virus Replication in Mouse Macrophages

<p>RAW 264.7 cells (mouse peritoneal macrophage-derived cell line) were infected with a multiplicity of infection of 0.05. Cells were untreated (A), treated with murine IFN-α/β (500 units/ml) 2 h postinfection (B), or treated with murine IFN-α/β (500 units/ml) 12 h prior to and again 2 h after virus adsorption (C). Supernatants were collected on days 0, 1, 2, 3, and 4 postinfection and titrated by use of a focus-forming unit assay in Vero E6 cells [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.0020073#ppat-0020073-b036" target="_blank">36</a>].