Expression Study Results for the <i>Bällchen and Hun</i> gene

<div><p>(A) <i>Bällchen</i> is expressed ubiquitously in both <i>D.melanogaster</i> and <i>D. simulans:</i> whole body, D. melanogaster (1), D. simulans (2); head, D. melanogaster (3), D. simulans (4); thorax, D. melanogaster (5), <i>D. simulans</i> (6); testes, D. melanogaster (7), D. simulans (8); and accessory gland with ejaculatory duct, D. melanogaster (9), D. simulans (10). Lane 11 is a genomic control.</p><p>(B) RT-PCR results for <i>Hun</i> from adult male and female <i>D. simulans, D. mauritiana,</i> and D. sechellia flies. <i>Hun</i>'s expression is limited to <i>D. simulans, D. mauritiana,</i> and D. sechellia males, lanes 1, 5, and 9, respectively. Lanes 3, 7, and 11 are (D) <i>D. simulans, D. mauritiana,</i> and D. sechellia females. Lanes 2, 4, 6, 8, 10, and 12 are corresponding RT-controls. (C–E) Tissue specific RT-PCR results for the <i>Hun</i> gene in D. simulans (C), D. mauritiana (D), and D. sechellia (E). For these RT-PCR experiments testes were dissected from the rest of body. <i>Hun</i>'s expression is testes-specific for each species: testes, lane 1; RT-control, lane 2; <i>Gapdh-2</i> positive control, lane 3; rest of body, lane 4; RT-control, lane 5; <i>Gapdh-2</i> positive control, lane 6.</p>