<div><p>(A) Wnt5a-mediated inhibition is PTX insensitive. At 24 h post-transfection, 293 cells were pretreated with vehicle or 100 ng/ml PTX for 24 h. Cells were then treated with indicated Wnt proteins concomitantly with vehicle or PTX for an additional 24 h, and luciferase assay was performed.</p> <p>(B) PTX is active in 293 cells as PTX pretreatment of cells inhibits LPA-induced Erk1,2 activation. The 293 cells were pretreated with 100 ng/ml PTX for 24 h and then treated with 10 μM LPA or vehicle for 10 min. Western blot analysis was then performed on total cell lysates using anti–phospho-Erk1,2 antibody. Membrane was stripped and reprobed for total Erk1,2 as a loading control.</p> <p>(C) Wnt5a protein does not di...
<p><b>Copyright information:</b></p><p>Taken from "Phosphoprotein phosphatase-2A docks to Dishevelle...
<p><b>A.</b> Expression of endogenous and recombinant Wnt5a in whole cell lysates of stably transfec...
<p><b>Copyright information:</b></p><p>Taken from "Phosphoprotein phosphatase-2A docks to Dishevelle...
<div><p>(A) Wnt5a treatment leads to β-catenin stabilization specifically in cells expressing mFz4. ...
<p>Both WNT5A proteins block Wnt3a-activation of β-catenin/TCF-driven transcriptional activity in HE...
<div><p>(A) Coomassie blue–stained gel showing the Wnt5a protein purification scheme.</p> ...
<p>A) For studying long-range signaling, Xwnt5A-EGFP transfected HEK293 cells seeded on thinCerts TC...
Wnt5a is a non-canonical signaling Wnt that has been implicated in tumor suppression. We previously ...
Wnt5a is a non-canonical signaling Wnt that has been implicated in tumor suppression. We previously ...
<p><b>Panel A:</b> L S/L cells were transiently transfected with Wnt1. 4 hrs post-transfection, the ...
<p>(<i>A</i>, <i>B</i>,) GCPs were treated with WNT3 for 24 h and lysates analyzed by Western blot a...
<p><b>signaling is not involved in US28-mediated Tcf-Lef activation.</b> A, Western blot analysis of...
<p>(A, B) MDA-MB-231 cells were stimulated by rWnt5a at the indicated doses for 4 h. The cell motili...
<p><b>A</b>. The expression of the WNT target gene: <i>AXIN2</i> was determined from NIH 3T3 cells e...
<p>(a) TCF/LEF luciferase reporter assay in A549 cells. To characterize the sLRP6E1E2 effects on the...
<p><b>Copyright information:</b></p><p>Taken from "Phosphoprotein phosphatase-2A docks to Dishevelle...
<p><b>A.</b> Expression of endogenous and recombinant Wnt5a in whole cell lysates of stably transfec...
<p><b>Copyright information:</b></p><p>Taken from "Phosphoprotein phosphatase-2A docks to Dishevelle...
<div><p>(A) Wnt5a treatment leads to β-catenin stabilization specifically in cells expressing mFz4. ...
<p>Both WNT5A proteins block Wnt3a-activation of β-catenin/TCF-driven transcriptional activity in HE...
<div><p>(A) Coomassie blue–stained gel showing the Wnt5a protein purification scheme.</p> ...
<p>A) For studying long-range signaling, Xwnt5A-EGFP transfected HEK293 cells seeded on thinCerts TC...
Wnt5a is a non-canonical signaling Wnt that has been implicated in tumor suppression. We previously ...
Wnt5a is a non-canonical signaling Wnt that has been implicated in tumor suppression. We previously ...
<p><b>Panel A:</b> L S/L cells were transiently transfected with Wnt1. 4 hrs post-transfection, the ...
<p>(<i>A</i>, <i>B</i>,) GCPs were treated with WNT3 for 24 h and lysates analyzed by Western blot a...
<p><b>signaling is not involved in US28-mediated Tcf-Lef activation.</b> A, Western blot analysis of...
<p>(A, B) MDA-MB-231 cells were stimulated by rWnt5a at the indicated doses for 4 h. The cell motili...
<p><b>A</b>. The expression of the WNT target gene: <i>AXIN2</i> was determined from NIH 3T3 cells e...
<p>(a) TCF/LEF luciferase reporter assay in A549 cells. To characterize the sLRP6E1E2 effects on the...
<p><b>Copyright information:</b></p><p>Taken from "Phosphoprotein phosphatase-2A docks to Dishevelle...
<p><b>A.</b> Expression of endogenous and recombinant Wnt5a in whole cell lysates of stably transfec...
<p><b>Copyright information:</b></p><p>Taken from "Phosphoprotein phosphatase-2A docks to Dishevelle...